BACKGROUND: Several reports have previously suggested that oligomineral water may have a beneficial immunomodulatory role in skin physiology. However, molecular, and cellular mechanisms through which oligo-elements act in cutaneous trophism have not yet been fully clari- fied. Among the external stimuli that affect the skin, ultraviolet (UV) radiation, which is frequently encountered in everyday life, is a major envi- ronmental factor of skin damage. Keratinocytes are the major target of UV, and they play a key role in a first line of body defenses. Accumulating evidence suggests that UVB irradiation induces nuclear DNA damage, membrane destruction, resulting in apoptosis and skin inflammation. The aim of this study was to investigate the anti-inflammatory, antioxidant, antiapoptotic effects of Rocchetta® oligomineral (Co.Ge.Di. International SpA, Rome, Italy) water in UVB-irradiated immortalized human keratinocytes.
METHODS: HaCaT UVB-irradiated was cultured with increasing concentrations of Rocchetta® oligomineral water. To evaluate the anti-inflam- matory properties gene expression of TNF, IL1β, IL6, COX2 and Caspase1 was performed. Moreover, the antiapoptotic effects were evaluated through gene expression of GADD45, Caspase3 and RIPK3. Finally, we evaluated the antioxidant activity of Rocchetta® oligomineral water by measuring total ROS/RNS and superoxide production as markers of oxidative stress after UVB irradiation.
RESULTS: Our findings have shown that Rocchetta® oligomineral water is well tolerated by the cells and displays anti-inflammatory, antioxi- dant and antiapoptotic proprieties when used prior keratinocyte UVB irradiation.
CONCLUSIONS: Our results highlight a possible protective role of Rocchetta oligomineral water in modulating the cutaneous inflammatory response to external triggers and injuries.
(Cite this article as: Caiazzo G, Parisi M, Luciano MA, Di Caprio R, Gallo L, Cacciapuoti S, et al. Beneficial effects of Rocchetta® oligomineral water in HaCaT keratinocytes after ultraviolet-B irradiation. Ital J Dermatol Venereol 2022;157. DOI: 10.23736/S2784-8671.22.07251-6)
Key words: Drinking water; Keratinocytes; Ultraviolet therapy; Anti-inflammatory agents; Antioxidants.
Giuseppina CAIAZZO, Melania PARISI, Maria A. LUCIANO, Roberta DI CAPRIO, Lucia GALLO, Sara CACCIAPUOTI, Maria QUARANTA, Gabriella FABBROCINI
Section of Dermatology, Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy
Department of Public Health, University of Naples Federico II, Naples, Italy